› 6tro

X-ray diffraction
3Å resolution

Crystal structure of the T-cell receptor GVY01 bound to HLA A2*01-GVYDGREHTV

Released:
DOI: 10.2210/pdb6tro/pdb
PDB entry 6tro coloured by chain, front view.
PDB entry 6tro coloured by chain, side view.
PDB entry 6tro coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
T cell receptor interactions with human leukocyte antigen govern indirect peptide selectivity for the cancer testis antigen MAGE-A4.
Coles CH, McMurran C, Lloyd A, Hock M, Hibbert L, Raman MCC, Hayes C, Lupardus P, Cole DK, Harper S
J Biol Chem 295 11486-11494 (2020)
PMID: 32532817

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-211239 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53Z42 (Residues: 25-300; Coverage: 81%)
Gene name: HLA-A
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Melanoma-associated antigen 4 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P43358 (Residues: 230-239; Coverage: 3%)
Gene names: MAGE4, MAGEA4
T-cell receptor alpha chain Chain: D
Molecule details ›
Chain: D
Length: 208 amino acids
Theoretical weight: 23.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
T-cell receptor beta chain Chain: E
Molecule details ›
Chain: E
Length: 247 amino acids
Theoretical weight: 28.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P6422
Unit cell:
a: 220.04Å b: 220.04Å c: 96.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.213 0.27
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

HLA-A29 and Birdshot Uveitis: Further Down the Rabbit Hole.
Kuiper et al. (2020)
3 more

5 mentions without citation

TCR Recognition of Peptide-MHC-I: Rule Makers and Breakers.
Szeto et al. (2020)
4 more