› 6tzb

X-ray diffraction
2.24Å resolution

Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin in complex with 6'-SLNLN

Released:
DOI: 10.2210/pdb6tzb/pdb
PDB entry 6tzb coloured by chain, front view.
PDB entry 6tzb coloured by chain, side view.
PDB entry 6tzb coloured by chain, top view.
Source organism: Influenza A virus (A/Hong Kong/1/1968(H3N2))
Primary publication:
Major antigenic site B of human influenza H3N2 viruses has an evolving local fitness landscape.
Wu NC, Otwinowski J, Thompson AJ, Nycholat CM, Nourmohammad A, Wilson IA
Nat Commun 11 1233 (2020)
PMID: 32144244

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124972 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (8 distinct):
Hemagglutinin HA1 chain Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 321 amino acids
Theoretical weight: 35.32 KDa
Source organism: Influenza A virus (A/Hong Kong/1/1968(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q91MA7 (Residues: 27-345; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 176 amino acids
Theoretical weight: 20.2 KDa
Source organism: Influenza A virus (A/Hong Kong/1/1968(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: H9XC94 (Residues: 346-521; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 210.401Å b: 132.173Å c: 72.468Å
α: 90° β: 97.93° γ: 90°
R-values:
R R work R free
0.16 0.157 0.207
Expression system: Trichoplusia ni

Quick links

Citations

11 review citations

HIV-1 and SARS-CoV-2: Patterns in the evolution of two pandemic pathogens.
Fischer et al. (2021)
10 more

3 mentions without citation

Adaptation of influenza viruses to human airway receptors.
Thompson et al. (2021)
2 more