› 6u0e

Hybrid

Neutron crystal structure of T4L M6AE

Released:
DOI: 10.2210/pdb6u0e/pdb
PDB entry 6u0e coloured by chain, front view.
PDB entry 6u0e coloured by chain, side view.
PDB entry 6u0e coloured by chain, top view.
Source organism: Escherichia virus T4
Entry authors: Cuneo MJ, Myles DA, Li L

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.57 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: D9IEF7 (Residues: 1-164; Coverage: 100%)
Gene names: T4Tp126, e
Sequence domains: Phage lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Experimental Method: Neutron Diffraction, X-ray diffraction
X-ray source: RIGAKU MICROMAX-007 HF, ORNL Spallation Neutron Source BEAMLINE MANDI
Spacegroup: P3221
Unit cell:
a: 60.8Å b: 60.8Å c: 97.07Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.184 0.235
Expression system: Escherichia coli K-12

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