› 6u0m

Electron Microscopy
3.9Å resolution

Structure of the S. cerevisiae replicative helicase CMG in complex with a forked DNA

Released:
DOI: 10.2210/pdb6u0m/pdb
PDB entry 6u0m coloured by chain, front view.
PDB entry 6u0m coloured by chain, side view.
PDB entry 6u0m coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
DNA unwinding mechanism of a eukaryotic replicative CMG helicase.
Yuan Z, Georgescu R, Bai L, Zhang D, Li H, O'Donnell ME
Nat Commun 11 688- (2020)
Related structures: EMD-20607

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120666 (preferred)
Entry contents:
11 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (13 distinct):
DNA replication complex GINS protein PSF1 Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 24.23 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12488 (Residues: 1-208; Coverage: 100%)
Gene names: PSF1, PZA208, YD8119.18, YDR013W
Sequence domains: GINS complex protein
DNA replication complex GINS protein PSF2 Chain: B
Molecule details ›
Chain: B
Length: 198 amino acids
Theoretical weight: 23.43 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40359 (Residues: 3-200; Coverage: 93%)
Gene names: HRF213, J1086, PSF2, YJL072C
Sequence domains: GINS complex protein
DNA replication complex GINS protein PSF3 Chain: C
Molecule details ›
Chain: C
Length: 191 amino acids
Theoretical weight: 21.66 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12146 (Residues: 3-193; Coverage: 99%)
Gene names: PSF3, YOL146W
Sequence domains: GINS complex protein
DNA replication complex GINS protein SLD5 Chain: D
Molecule details ›
Chain: D
Length: 291 amino acids
Theoretical weight: 33.62 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03406 (Residues: 3-293; Coverage: 99%)
Gene names: SLD5, YDR489W
Sequence domains:
Cell division control protein 45 Chain: E
Molecule details ›
Chain: E
Length: 646 amino acids
Theoretical weight: 73.81 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q08032 (Residues: 5-650; Coverage: 99%)
Gene names: CDC45, L8004.11, SLD4, YLR103C
Sequence domains: CDC45-like protein
DNA replication licensing factor MCM2 Chain: 2
Molecule details ›
Chain: 2
Length: 664 amino acids
Theoretical weight: 74.99 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29469 (Residues: 201-864; Coverage: 77%)
Gene names: MCM2, YBL023C, YBL0438
Sequence domains:
DNA replication licensing factor MCM3 Chain: 3
Molecule details ›
Chain: 3
Length: 722 amino acids
Theoretical weight: 79.99 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P24279 (Residues: 17-738; Coverage: 74%)
Gene names: MCM3, SYGP-ORF23, YEL032W
Sequence domains:
DNA replication licensing factor MCM4 Chain: 4
Molecule details ›
Chain: 4
Length: 753 amino acids
Theoretical weight: 85.82 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P30665 (Residues: 177-929; Coverage: 81%)
Gene names: CDC54, HCD21, MCM4, YP9531.13, YPR019W
Sequence domains:
Minichromosome maintenance protein 5 Chain: 5
Molecule details ›
Chain: 5
Length: 670 amino acids
Theoretical weight: 74.4 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P29496 (Residues: 24-693; Coverage: 87%)
Gene names: CDC46, L9328.1, MCM5, YLR274W
Sequence domains:
DNA replication licensing factor MCM6 Chain: 6
Molecule details ›
Chain: 6
Length: 667 amino acids
Theoretical weight: 73.93 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P53091 (Residues: 103-840; Coverage: 66%)
Gene names: MCM6, YGL201C
Sequence domains:
DNA replication licensing factor MCM7 Chain: 7
Molecule details ›
Chain: 7
Length: 729 amino acids
Theoretical weight: 82.03 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38132 (Residues: 1-729; Coverage: 86%)
Gene names: CDC47, MCM7, YBR1441, YBR202W
Sequence domains:
DNA (26-MER) Chain: F
Molecule details ›
Chain: F
Length: 23 nucleotides
Theoretical weight: 7.08 KDa
DNA (15-MER) Chain: G
Molecule details ›
Chain: G
Length: 15 nucleotides
Theoretical weight: 4.59 KDa

Ligands and Environments

1 bound ligand:
Ligand ATP 3 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-20607
Expression system: Saccharomyces cerevisiae

Quick links

Citations

16 review citations

Mechanisms of hexameric helicases.
Fernandez et al. (2021)
15 more

4 mentions without citation

Mechanism of replication origin melting nucleated by CMG helicase assembly.
Lewis et al. (2022)
3 more