› 6u18

X-ray diffraction
2Å resolution

Directed evolution of a biosensor selective for the macrolide antibiotic clarithromycin

Released:
DOI: 10.2210/pdb6u18/pdb
PDB entry 6u18 coloured by chain, front view.
PDB entry 6u18 coloured by chain, side view.
PDB entry 6u18 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Development of Genetically Encoded Biosensors for Reporting the Methyltransferase-Dependent Biosynthesis of Semisynthetic Macrolide Antibiotics.
Li Y, Reed M, Wright HT, Cropp TA, Williams GJ
ACS Synth Biol 10 2520-2531 (2021)
PMID: 34546703

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 21.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9EVJ6 (Residues: 1-194; Coverage: 100%)
Gene names: AKG29_00740, B6R15_004956, B6R15_005534, BANRA_05395, BE932_01835, BJI68_09320, BK292_28800, BK383_16415, D4U49_26715, D4U49_28860, DS732_30515, ETN48_p0081, G3V95_25045, G5603_26380, GRC73_23715, GRC73_24600, HL601_25835, HL601_27160, HMV95_24735, HMV95_26340, ID255_p0130, KKS54_p00365, OGM49_25985, RCS105_pI0107, RCS28_PI0043, RCS51_P0076, RCS55TR727_P0019, RCS57_p0098, RCS65_P0019, RCS78_P0035, RCS79_P0004, SAMEA4362930_00093, SAMEA4370330_00106, SAMEA4370365_00009, SAMEA4370473_00003, TF1-34_00092, mphR, mphR(A)
Sequence domains: Bacterial regulatory proteins, tetR family

Ligands and Environments

2 bound ligands:
Ligand CTY 2 x CTY
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 42.897Å b: 53.161Å c: 158.815Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Striving for sustainable biosynthesis: discovery, diversification, and production of antimicrobial drugs in Escherichia coli.
Iacovelli et al. (2022)
1 more

1 mention without citation

Development of Genetically Encoded Biosensors for Reporting the Methyltransferase-Dependent Biosynthesis of Semisynthetic Macrolide Antibiotics.
Li et al. (2021)