› 6u2m

X-ray diffraction
2Å resolution

Crystal structure of a HaloTag-based calcium indicator, HaloCaMP V2, bound to JF635

Released:
DOI: 10.2210/pdb6u2m/pdb
PDB entry 6u2m coloured by chain, front view.
PDB entry 6u2m coloured by chain, side view.
PDB entry 6u2m coloured by chain, top view.
Source organism: Rhodococcus sp.
Primary publication:
The HaloTag as a general scaffold for far-red tunable chemigenetic indicators.
Deo C, Abdelfattah AS, Bhargava HK, Berro AJ, Falco N, Farrants H, Moeyaert B, Chupanova M, Lavis LD, Schreiter ER
Nat Chem Biol 17 718-723 (2021)
PMID: 33795886

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HaloCaMP V2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 498 amino acids
Theoretical weight: 55.85 KDa
Source organism: Rhodococcus sp.
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand PUJ 2 x PUJ
Ligand CL 2 x CL
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P2
Unit cell:
a: 92.56Å b: 60.662Å c: 122.6Å
α: 90° β: 91.02° γ: 90°
R-values:
R R work R free
0.188 0.186 0.226
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels.
Grimm et al. (2022)
11 more

1 mention without citation

The design and evolution of fluorescent protein-based sensors for monoatomic ions in biology.
Baek et al. (2021)