› 6u3j

X-ray diffraction
2.25Å resolution

Structure of the 2-oxoadipate dehydrogenase DHTKD1

Released:
DOI: 10.2210/pdb6u3j/pdb
PDB entry 6u3j coloured by chain, front view.
PDB entry 6u3j coloured by chain, side view.
PDB entry 6u3j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inhibition and Crystal Structure of the Human DHTKD1-Thiamin Diphosphate Complex.
Leandro J, Khamrui S, Wang H, Suebsuwong C, Nemeria NS, Huynh K, Moustakim M, Secor C, Wang M, Dodatko T, Stauffer B, Wilson CG, Yu C, Arkin MR, Jordan F, Sanchez R, DeVita RJ, Lazarus MB, Houten SM
ACS Chem Biol 15 2041-2047 (2020)
PMID: 32633484

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-oxoadipate dehydrogenase complex component E1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 904 amino acids
Theoretical weight: 101.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96HY7 (Residues: 25-919; Coverage: 97%)
Gene names: DHTKD1, KIAA1630
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand TPP 2 x TPP
Ligand MG 3 x MG
1 modified residue:
Ligand MLZ 4 x MLZ

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: C2
Unit cell:
a: 332.314Å b: 72.612Å c: 79.675Å
α: 90° β: 91.87° γ: 90°
R-values:
R R work R free
0.211 0.209 0.241
Expression system: Escherichia coli

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Citations

2 review citations

Rare Disease Registries Are Key to Evidence-Based Personalized Medicine: Highlighting the European Experience.
Kölker et al. (2022)
1 more

3 mentions without citation

Toward an Understanding of the Structural and Mechanistic Aspects of Protein-Protein Interactions in 2-Oxoacid Dehydrogenase Complexes.
Nemeria et al. (2021)
2 more