› 6u55

X-ray diffraction
1.93Å resolution

Anti-Zaire ebolavirus Nucleoprotein Single Domain Antibody Zaire E (ZE) Complexed with Sudan ebolavirus Nucleoprotein C-terminal Domain 634-738

Released:
DOI: 10.2210/pdb6u55/pdb
PDB entry 6u55 coloured by chain, front view.
PDB entry 6u55 coloured by chain, side view.
PDB entry 6u55 coloured by chain, top view.
Source organisms:
Primary publication:
Paratope Duality and Gullying are Among the Atypical Recognition Mechanisms Used by a Trio of Nanobodies to Differentiate Ebolavirus Nucleoproteins.
Sherwood LJ, Taylor AB, Hart PJ, Hayhurst A
J Mol Biol 431 4848-4867 (2019)
PMID: 31626803

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Anti-Zaire ebolavirus Nucleoprotein Single Domain Antibody Zaire E (ZE) Chain: A
Molecule details ›
Chain: A
Length: 117 amino acids
Theoretical weight: 12.69 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Nucleoprotein Chain: B
Molecule details ›
Chain: B
Length: 117 amino acids
Theoretical weight: 13.59 KDa
Source organism: Sudan virus - Boniface, Sudan,1976
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9QP77 (Residues: 632-738; Coverage: 15%)
Gene name: NP

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 93.398Å b: 28.298Å c: 86.541Å
α: 90° β: 114.453° γ: 90°
R-values:
R R work R free
0.2 0.192 0.263
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Toolkit for Quickly Generating and Characterizing Molecular Probes Specific for SARS-CoV-2 Nucleocapsid as a Primer for Future Coronavirus Pandemic Preparedness.
Sherwood et al. (2021)
1 more

1 mention without citation

Paratope Duality and Gullying are Among the Atypical Recognition Mechanisms Used by a Trio of Nanobodies to Differentiate Ebolavirus Nucleoproteins.
Sherwood et al. (2019)