› 6u6u

X-ray diffraction
2.31Å resolution

IL36R extracellular domain in complex with BI655130 Fab

Released:
DOI: 10.2210/pdb6u6u/pdb
PDB entry 6u6u coloured by chain, front view.
PDB entry 6u6u coloured by chain, side view.
PDB entry 6u6u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray crystal structure localizes the mechanism of inhibition of an IL-36R antagonist monoclonal antibody to interaction with Ig1 and Ig2 extra cellular domains.
Larson ET, Brennan DL, Hickey ER, Ganesan R, Kroe-Barrett R, Farrow NA
Protein Sci 29 1679-1686 (2020)
PMID: 32239732

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232179 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
BI00655130 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.78 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
BI00655130 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.64 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Interleukin-1 receptor-like 2 Chain: R
Molecule details ›
Chain: R
Length: 197 amino acids
Theoretical weight: 22.39 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9HB29 (Residues: 21-215; Coverage: 35%)
Gene names: IL1RL2, IL1RRP2
Sequence domains: Immunoglobulin domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NA 1 x NA
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 60.698Å b: 70.663Å c: 233.268Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.202
Expression systems:
  • Cricetulus griseus
  • Homo sapiens

Quick links

Citations

1 review citation

Interleukin-36 Cytokine/Receptor Signaling: A New Target for Tissue Fibrosis.
Melton et al. (2020)

1 mention without citation

X-ray crystal structure localizes the mechanism of inhibition of an IL-36R antagonist monoclonal antibody to interaction with Ig1 and Ig2 extra cellular domains.
Larson et al. (2020)