› 6u9d

X-ray diffraction
3.19Å resolution

Saccharomyces cerevisiae acetohydroxyacid synthase

Released:
DOI: 10.2210/pdb6u9d/pdb
PDB entry 6u9d coloured by chain, front view.
PDB entry 6u9d coloured by chain, side view.
PDB entry 6u9d coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structures of fungal and plant acetohydroxyacid synthases.
Lonhienne T, Low YS, Garcia MD, Croll T, Gao Y, Wang Q, Brillault L, Williams CM, Fraser JA, McGeary RP, West NP, Landsberg MJ, Rao Z, Schenk G, Guddat LW
Nature 586 317-321 (2020)
PMID: 32640464

Function and Biology Details

Reaction catalysed: 
2 pyruvate = 2-acetolactate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139530 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acetolactate synthase catalytic subunit, mitochondrial Chains: A, B, E, F, I, J, M, N, Q, R, U, V
Molecule details ›
Chains: A, B, E, F, I, J, M, N, Q, R, U, V
Length: 644 amino acids
Theoretical weight: 70.26 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P07342 (Residues: 58-687; Coverage: 92%)
Gene names: ILV2, SMR1, YM9718.07, YMR108W
Sequence domains:
Acetolactate synthase small subunit, mitochondrial Chains: C, D, G, H, K, L, O, P, S, T, W, X
Molecule details ›
Chains: C, D, G, H, K, L, O, P, S, T, W, X
Length: 297 amino acids
Theoretical weight: 32.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P25605 (Residues: 41-309; Coverage: 87%)
Gene names: ILV6, YCL009C, YCL9C
Sequence domains:

Ligands and Environments


Cofactor: Ligand TPP 11 x TPP

Cofactor: Ligand FAD 12 x FAD
3 bound ligands:
Ligand MG 16 x MG
Ligand 60G 12 x 60G
Ligand ATP 12 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: C2
Unit cell:
a: 368.647Å b: 230.307Å c: 183.532Å
α: 90° β: 94.57° γ: 90°
R-values:
R R work R free
0.205 0.205 0.252
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins.
Priest et al. (2021)
3 more

1 mention without citation

Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.
Naz et al. (2023)