› 6u9o

Electron Microscopy
3.36Å resolution

Human IMPDH2 treated with ATP, IMP, NAD+, and 2 mM GTP. Fully compressed filament segment reconstruction.

Released:
DOI: 10.2210/pdb6u9o/pdb
PDB entry 6u9o coloured by chain, front view.
PDB entry 6u9o coloured by chain, side view.
PDB entry 6u9o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures demonstrate human IMPDH2 filament assembly tunes allosteric regulation.
Johnson MC, Kollman JM
Elife 9 (2020)
PMID: 31999252
Related structures: EMD-20701

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase 2 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 519 amino acids
Theoretical weight: 56.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12268 (Residues: 1-514; Coverage: 100%)
Gene names: IMPD2, IMPDH2
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
3 bound ligands:
Ligand GTP 16 x GTP
Ligand ATP 8 x ATP
Ligand IMP 8 x IMP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.36Å
Relevant EMDB volumes: EMD-20701
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Targeting cancer metabolism in the era of precision oncology.
Stine et al. (2022)
5 more

3 mentions without citation

IMPDH1 retinal variants control filament architecture to tune allosteric regulation.
Burrell et al. (2022)
2 more