› 6u9p

X-ray diffraction
1.65Å resolution

Wild-type MthK pore in ~150 mM K+

Released:
DOI: 10.2210/pdb6u9p/pdb
PDB entry 6u9p coloured by chain, front view.
PDB entry 6u9p coloured by chain, side view.
PDB entry 6u9p coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
Selectivity filter ion binding affinity determines inactivation in a potassium channel.
Boiteux C, Posson DJ, Allen TW, Nimigean CM
Proc Natl Acad Sci U S A 117 29968-29978 (2020)
PMID: 33154158

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127898 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-gated potassium channel MthK Chain: A
Molecule details ›
Chain: A
Length: 82 amino acids
Theoretical weight: 8.98 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O27564 (Residues: 18-99; Coverage: 24%)
Gene names: MTH_1520, mthK
Sequence domains: Ion channel

Ligands and Environments

2 bound ligands:
Ligand K 5 x K
Ligand HEZ 1 x HEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P4212
Unit cell:
a: 63.811Å b: 63.811Å c: 44.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.208
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Molecular Mechanisms for Bacterial Potassium Homeostasis.
Stautz et al. (2021)
1 more