› 6ucu

Electron Microscopy
3.06Å resolution

Cryo-EM structure of the mitochondrial TOM complex from yeast (dimer)

Released:
DOI: 10.2210/pdb6ucu/pdb
PDB entry 6ucu coloured by chain, front view.
PDB entry 6ucu coloured by chain, side view.
PDB entry 6ucu coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cryo-EM structure of the mitochondrial protein-import channel TOM complex at near-atomic resolution.
Tucker K, Park E
Nat Struct Mol Biol 26 1158-1166 (2019)
PMID: 31740857
Related structures: EMD-20728

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150030 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Mitochondrial import receptor subunit TOM40 Chains: A, I
Molecule details ›
Chains: A, I
Length: 397 amino acids
Theoretical weight: 43.23 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P23644 (Residues: 1-387; Coverage: 100%)
Gene names: ISP42, MOM38, TOM40, YM8325.04, YMR203W
Sequence domains: Eukaryotic porin
Mitochondrial import receptor subunit TOM22 Chains: B, J
Molecule details ›
Chains: B, J
Length: 162 amino acids
Theoretical weight: 18.02 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P49334 (Residues: 1-152; Coverage: 100%)
Gene names: MAS17, MAS22, MOM22, N1217, N1862, TOM22, YNL131W
Sequence domains: Mitochondrial import receptor subunit Tom22
Mitochondrial import receptor subunit TOM5 Chains: C, K
Molecule details ›
Chains: C, K
Length: 50 amino acids
Theoretical weight: 5.99 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P80967 (Residues: 1-50; Coverage: 100%)
Gene names: MOM8A, TOM5, YPR133W-A
Sequence domains: Mitochondrial import receptor subunit or translocase
Mitochondrial import receptor subunit TOM6 Chains: D, L
Molecule details ›
Chains: D, L
Length: 61 amino acids
Theoretical weight: 6.41 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P33448 (Residues: 1-61; Coverage: 100%)
Gene names: ISP6, TOM6, YOR045W
Sequence domains: Mitochondrial import receptor subunit Tom6, fungal
Mitochondrial import receptor subunit TOM7 Chains: E, M
Molecule details ›
Chains: E, M
Length: 60 amino acids
Theoretical weight: 6.88 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P53507 (Residues: 1-60; Coverage: 100%)
Gene names: MOM7, N2378, TOM7, YNL070W
Sequence domains: TOM7 family

Ligands and Environments

1 bound ligand:
Ligand LMT 60 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.06Å
Relevant EMDB volumes: EMD-20728
Expression system: Saccharomyces cerevisiae S288C

Quick links

Citations

31 review citations

Quality control of the mitochondrial proteome.
Song et al. (2021)
30 more

10 mentions without citation

A Biochemical and Structural Understanding of TOM Complex Interactions and Implications for Human Health and Disease.
Pitt et al. (2021)
9 more