› 6uej

X-ray diffraction
2.21Å resolution

Crystal structure of human zinc finger antiviral protein bound to RNA

Released:
DOI: 10.2210/pdb6uej/pdb
PDB entry 6uej coloured by chain, front view.
PDB entry 6uej coloured by chain, side view.
PDB entry 6uej coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the zinc-finger antiviral protein in complex with RNA reveals a mechanism for selective targeting of CG-rich viral sequences.
Meagher JL, Takata M, Gonçalves-Carneiro D, Keane SC, Rebendenne A, Ong H, Orr VK, MacDonald MR, Stuckey JA, Bieniasz PD, Smith JL
Proc Natl Acad Sci U S A 116 24303-24309 (2019)
PMID: 31719195

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182241 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Zinc finger CCCH-type antiviral protein 1 Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 25.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z2W4 (Residues: 2-227; Coverage: 25%)
Gene names: PRO1677, ZC3HAV1, ZC3HDC2
Sequence domains:
RNA (5'-R(P*UP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 3 nucleotides
Theoretical weight: 912 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand SPM 1 x SPM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P65
Unit cell:
a: 123.09Å b: 123.09Å c: 40.67Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.211 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

26 review citations

The Potential Intermediate Hosts for SARS-CoV-2.
Zhao et al. (2020)
25 more

3 mentions without citation

Structure of the zinc-finger antiviral protein in complex with RNA reveals a mechanism for selective targeting of CG-rich viral sequences.
Meagher et al. (2019)
2 more