› 6ufm

X-ray diffraction
2.82Å resolution

Cocrystal Structure of the Nocardia farcinica ileS T-box riboswitch in complex with its cognate tRNA

Released:
DOI: 10.2210/pdb6ufm/pdb
PDB entry 6ufm coloured by chain, front view.
PDB entry 6ufm coloured by chain, side view.
PDB entry 6ufm coloured by chain, top view.
Source organisms:
Primary publication:
High-affinity recognition of specific tRNAs by an mRNA anticodon-binding groove.
Suddala KC, Zhang J
Nat Struct Mol Biol 26 1114-1122 (2019)
PMID: 31792448

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195831 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (77-MER) Chain: A
Molecule details ›
Chain: A
Length: 77 nucleotides
Theoretical weight: 24.85 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Not provided
Sequence domains: tRNA
RNA (98-MER) Chain: B
Molecule details ›
Chain: B
Length: 98 nucleotides
Theoretical weight: 31.78 KDa
Source organism: Nocardia farcinica
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P2221
Unit cell:
a: 42.23Å b: 93.98Å c: 179.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.191 0.233
Expression system: Not provided

Quick links

Citations

10 review citations

Advances in RNA 3D Structure Modeling Using Experimental Data.
Li et al. (2020)
9 more

7 mentions without citation

Unboxing the T-box riboswitches-A glimpse into multivalent and multimodal RNA-RNA interactions.
Zhang J. (2020)
6 more