› 6uh8

X-ray diffraction
1.58Å resolution

Crystal structure of DAD2 N242I mutant

Released:
DOI: 10.2210/pdb6uh8/pdb
PDB entry 6uh8 coloured by chain, front view.
PDB entry 6uh8 coloured by chain, side view.
PDB entry 6uh8 coloured by chain, top view.
Source organism: Petunia x hybrida
Primary publication:
Flexibility of the petunia strigolactone receptor DAD2 promotes its interaction with signaling partners.
Lee HW, Sharma P, Janssen BJ, Drummond RSM, Luo Z, Hamiaux C, Collier T, Allison JR, Newcomb RD, Snowden KC
J Biol Chem 295 4181-4193 (2020)
PMID: 32071083

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-125406 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable strigolactone esterase DAD2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.89 KDa
Source organism: Petunia x hybrida
Expression system: Escherichia coli
UniProt:
  • Canonical: J9U5U9 (Residues: 1-267; Coverage: 100%)
Gene name: DAD2
Sequence domains: Alpha/beta hydrolase family

Ligands and Environments

4 bound ligands:
Ligand MES 2 x MES
Ligand MG 7 x MG
Ligand GOL 2 x GOL
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P65
Unit cell:
a: 133.736Å b: 133.736Å c: 99.764Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.14 0.138 0.165
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The mechanism of host-induced germination in root parasitic plants.
Nelson DC. (2021)
1 more

1 mention without citation

Flexibility of the petunia strigolactone receptor DAD2 promotes its interaction with signaling partners.
Lee et al. (2020)