› 6uhc

Electron Microscopy
3.9Å resolution

CryoEM structure of human Arp2/3 complex with bound NPFs

Released:
DOI: 10.2210/pdb6uhc/pdb
PDB entry 6uhc coloured by chain, front view.
PDB entry 6uhc coloured by chain, side view.
PDB entry 6uhc coloured by chain, top view.
Source organisms:
Primary publication:
Zimmet A, Van Eeuwen T, Boczkowska M, Rebowski G, Murakami K, Dominguez R
Sci Adv
PMID: 32548263
Related structures: EMD-20770

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127308 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Actin-related protein 3 Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: P61158 (Residues: 1-418; Coverage: 100%)
Gene names: ACTR3, ARP3
Sequence domains: Actin
Actin-related protein 2 Chain: B
Molecule details ›
Chain: B
Length: 394 amino acids
Theoretical weight: 44.82 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: P61160 (Residues: 1-394; Coverage: 100%)
Gene names: ACTR2, ARP2
Sequence domains: Actin
Actin-related protein 2/3 complex subunit 1B Chain: C
Molecule details ›
Chain: C
Length: 372 amino acids
Theoretical weight: 41 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: O15143 (Residues: 1-372; Coverage: 100%)
Gene names: ARC41, ARPC1B
Sequence domains: WD domain, G-beta repeat
Actin-related protein 2/3 complex subunit 2 Chain: D
Molecule details ›
Chain: D
Length: 300 amino acids
Theoretical weight: 34.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: O15144 (Residues: 1-300; Coverage: 100%)
Gene names: ARC34, ARPC2, PRO2446
Sequence domains: Arp2/3 complex, 34 kD subunit p34-Arc
Actin-related protein 2/3 complex subunit 3 Chain: E
Molecule details ›
Chain: E
Length: 203 amino acids
Theoretical weight: 23.2 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: O15145 (Residues: 1-178; Coverage: 100%)
Gene names: ARC21, ARPC3
Sequence domains: ARP2/3 complex ARPC3 (21 kDa) subunit
Actin-related protein 2/3 complex subunit 4 Chain: F
Molecule details ›
Chain: F
Length: 168 amino acids
Theoretical weight: 19.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: P59998 (Residues: 1-168; Coverage: 100%)
Gene names: ARC20, ARPC4
Sequence domains: ARP2/3 complex 20 kDa subunit (ARPC4)
Actin-related protein 2/3 complex subunit 5 Chain: G
Molecule details ›
Chain: G
Length: 151 amino acids
Theoretical weight: 16.34 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 1 BOLD-2017
UniProt:
  • Canonical: O15511 (Residues: 1-151; Coverage: 100%)
Gene names: ARC16, ARPC5
Sequence domains: ARP2/3 complex 16 kDa subunit (p16-Arc)
Actin nucleation-promoting factor WASL Chains: H, I
Molecule details ›
Chains: H, I
Length: 501 amino acids
Theoretical weight: 54.34 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q91YD9 (Residues: 1-501; Coverage: 100%)
Gene name: Wasl
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-20770
Expression systems:
  • Spodoptera aff. frugiperda 1 BOLD-2017
  • Escherichia coli

Quick links

Citations

5 mentions without citation

Molecular mechanism of Arp2/3 complex inhibition by Arpin.
Fregoso et al. (2022)
4 more