› 6uig

X-ray diffraction
3.2Å resolution

Crystal structure of human monoclonal antibody H7.200 in complex with H7N9 hemagglutinin HA1

Released:
DOI: 10.2210/pdb6uig/pdb
PDB entry 6uig coloured by chain, front view.
PDB entry 6uig coloured by chain, side view.
PDB entry 6uig coloured by chain, top view.
Source organisms:
Primary publication:
Anti-influenza H7 human antibody targets antigenic site in hemagglutinin head domain interface.
Dong J, Gilchuk I, Li S, Irving R, Goff MT, Turner HL, Ward AB, Carnahan RH, Crowe JE
J Clin Invest 130 4734-4739 (2020)
PMID: 32749241

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232191 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Hemagglutinin Chains: A, G
Molecule details ›
Chains: A, G
Length: 308 amino acids
Theoretical weight: 33.76 KDa
Source organism: Influenza A virus
Expression system: Homo sapiens
UniProt:
  • Canonical: R4NN21 (Residues: 19-320; Coverage: 54%)
Gene name: HA
Sequence domains: Haemagglutinin
H7.200 Fab heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 227 amino acids
Theoretical weight: 24.27 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
H7.200 Fab light chain Chains: C, L
Molecule details ›
Chains: C, L
Length: 214 amino acids
Theoretical weight: 23.36 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P4322
Unit cell:
a: 156.493Å b: 156.493Å c: 229.084Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.207 0.234
Expression system: Homo sapiens

Quick links

Citations

3 review citations

Next-Generation Influenza HA Immunogens and Adjuvants in Pursuit of a Broadly Protective Vaccine.
Nagashima et al. (2021)
2 more

1 mention without citation

Award Winners and Abstracts of the 35th Anniversary Symposium of The Protein Society, July 7-14, 2021 | Virtual.
(2021)