› 6uk2

X-ray diffraction
3.14Å resolution

Complex of T cell Receptor with HHAT Wild Type Peptide KQWLVWLLL Presented by HLA-A206

Released:
DOI: 10.2210/pdb6uk2/pdb
PDB entry 6uk2 coloured by chain, front view.
PDB entry 6uk2 coloured by chain, side view.
PDB entry 6uk2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural dissimilarity from self drives neoepitope escape from immune tolerance.
Devlin JR, Alonso JA, Ayres CM, Keller GLJ, Bobisse S, Vander Kooi CW, Coukos G, Gfeller D, Harari A, Baker BM
Nat Chem Biol 16 1269-1276 (2020)
PMID: 32807968

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
PDBe Complex ID:
PDB-CPX-158326 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: U5YJP1 (Residues: 25-299; Coverage: 88%)
Gene name: HLA-A
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Protein-cysteine N-palmitoyltransferase HHAT Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.2 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q5VTY9 (Residues: 68-76; Coverage: 2%)
Gene names: HHAT, MART2, SKI1
302 TIL T cell receptor alpha chain Chain: D
Molecule details ›
Chain: D
Length: 208 amino acids
Theoretical weight: 23.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
302 TIL T cell receptor beta chain Chain: E
Molecule details ›
Chain: E
Length: 245 amino acids
Theoretical weight: 27.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 64.357Å b: 85.932Å c: 240.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.229 0.26
Expression systems:
  • Escherichia coli
  • Not provided
  • Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Identification of neoantigens for individualized therapeutic cancer vaccines.
Lang et al. (2022)
9 more

1 mention without citation

Structural dissimilarity from self drives neoepitope escape from immune tolerance.
Devlin et al. (2020)