› 6uki

X-ray diffraction
2.7Å resolution

HhaI endonuclease in Complex with DNA in space group P212121 (pH 6.0)

Released:
DOI: 10.2210/pdb6uki/pdb
Model geometry
Data not analysed
Fit model/data
Data not analysed
PDB entry 6uki coloured by chain, front view.
PDB entry 6uki coloured by chain, side view.
PDB entry 6uki coloured by chain, top view.
Source organism: Haemophilus parahaemolyticus
Primary publication:
Structure of HhaI endonuclease with cognate DNA at an atomic resolution of 1.0 Å.
Horton JR, Yang J, Zhang X, Petronzio T, Fomenkov A, Wilson GG, Roberts RJ, Cheng X
OpenAccess logo Nucleic Acids Res 48 1466-1478 (2020)
PMID: 31879785

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-244999 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HhaI Restriction Endonuclease Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 258 amino acids
Theoretical weight: 29.98 KDa
Source organism: Haemophilus parahaemolyticus
Expression system: Escherichia coli
DNA (5'-D(*CP*TP*GP*TP*TP*GP*CP*GP*CP*TP*TP*GP*GP*A)-3') Chains: A, D
Molecule details ›
Chains: A, D
Length: 14 nucleotides
Theoretical weight: 4.3 KDa
Source organism: Haemophilus parahaemolyticus
Expression system: Not provided
DNA (5'-D(*TP*CP*CP*AP*AP*GP*CP*GP*CP*AP*AP*CP*AP*G)-3') Chains: B, E

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 94.411Å b: 94.998Å c: 97.868Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.224
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 citation in other articles

Structural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P.
Zhu et al. (2022)
1 more

1 mention without citation

Structure of HhaI endonuclease with cognate DNA at an atomic resolution of 1.0 Å.
Horton et al. (2020)