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› 6ukj

Electron Microscopy
3.3Å resolution

Single-Particle Cryo-EM Structure of Plasmodium falciparum Chloroquine Resistance Transporter (PfCRT) 7G8 Isoform

Released:
DOI: 10.2210/pdb6ukj/pdb
PDB entry 6ukj coloured by chain, front view.
PDB entry 6ukj coloured by chain, side view.
PDB entry 6ukj coloured by chain, top view.
Source organisms:
Primary publication:
Structure and drug resistance of the Plasmodium falciparum transporter PfCRT.
Kim J, Tan YZ, Wicht KJ, Erramilli SK, Dhingra SK, Okombo J, Vendome J, Hagenah LM, Giacometti SI, Warren AL, Nosol K, Roepe PD, Potter CS, Carragher B, Kossiakoff AA, Quick M, Fidock DA, Mancia F
OpenAccess logo Nature 576 315-320 (2019)
PMID: 31776516
Related structures: EMD-20806

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212963 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Chloroquine resistance transporter Chain: A
Molecule details ›
Chain: A
Length: 464 amino acids
Theoretical weight: 53.07 KDa
Source organism: Plasmodium falciparum 7G8
Expression system: Homo sapiens
UniProt:
  • Canonical: W7FI62 (Residues: 2-424; Coverage: 100%)
Gene names: CG10, CRT, PFBG_01689
Sequence domains: CRT-like, chloroquine-resistance transporter-like
Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 241 amino acids
Theoretical weight: 25.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab Light Chain Chain: L

Ligands and Environments

1 bound ligand:
Ligand Y01 1 x Y01
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.3Å
Relevant EMDB volumes: EMD-20806
Expression systems:
  • Homo sapiens
  • Escherichia coli

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Citations

18 review citations

Molecular Mechanisms of Drug Resistance in Plasmodium falciparum Malaria.
Wicht et al. (2020)
17 more

15 mentions without citation

Cryo-electron microscopy analysis of small membrane proteins.
Nygaard et al. (2020)
14 more