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6ulg

Electron Microscopy
3.31Å resolution

Cryo-EM structure of the FLCN-FNIP2-Rag-Ragulator complex

Released:
Source organism: Homo sapiens
Primary publication:
Cryo-EM Structure of the Human FLCN-FNIP2-Rag-Ragulator Complex.
Cell 179 1319-1329.e8 (2019)
PMID: 31704029
Related structures: EMD-20814

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128944 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Folliculin Chain: L
Molecule details ›
Chain: L
Length: 579 amino acids
Theoretical weight: 64.55 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NFG4 (Residues: 1-579; Coverage: 100%)
Gene names: BHD, FLCN
Sequence domains:
Ragulator complex protein LAMTOR3 Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHA4 (Residues: 1-124; Coverage: 100%)
Gene names: LAMTOR3, MAP2K1IP1, MAPKSP1, PRO2783
Sequence domains: Mitogen-activated protein kinase kinase 1 interacting
Ragulator complex protein LAMTOR2 Chain: B
Ragulator complex protein LAMTOR5 Chain: C
Ragulator complex protein LAMTOR4 Chain: D
Ragulator complex protein LAMTOR1 Chain: E
Ras-related GTP-binding protein A Chain: F
Ras-related GTP-binding protein C Chain: G
Folliculin-interacting protein 2 Chain: N

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.31Å
Relevant EMDB volumes: EMD-20814
Expression systems:
  • Homo sapiens
  • Escherichia coli