› 6uli

X-ray diffraction
1.88Å resolution

Molecular basis for tumor infiltrating TCR recognition of hotspot KRAS-G12D mutation

Released:
DOI: 10.2210/pdb6uli/pdb
PDB entry 6uli coloured by chain, front view.
PDB entry 6uli coloured by chain, side view.
PDB entry 6uli coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-affinity oligoclonal TCRs define effective adoptive T cell therapy targeting mutant KRAS-G12D.
Sim MJW, Lu J, Spencer M, Hopkins F, Tran E, Rosenberg SA, Long EO, Sun PD
Proc Natl Acad Sci U S A 117 12826-12835 (2020)
PMID: 32461371

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-111132 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: C1K0Y1 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-C, HLA-Cw
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
GTPase NRas Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 762 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01111 (Residues: 10-18; Coverage: 5%)
Gene names: HRAS1, NRAS

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 95.365Å b: 76.266Å c: 62.696Å
α: 90° β: 118.219° γ: 90°
R-values:
R R work R free
0.2 0.199 0.226
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

24 review citations

Clinical implications of T cell exhaustion for cancer immunotherapy.
Chow et al. (2022)
23 more

3 mentions without citation

T Cell Recognition of Tumor Neoantigens and Insights Into T Cell Immunotherapy.
Sim et al. (2022)
2 more