› 6ulr

X-ray diffraction
3.2Å resolution

Molecular basis for tumor infiltrating TCR recognition of hotspot KRAS-G12D mutation

Released:
DOI: 10.2210/pdb6ulr/pdb
PDB entry 6ulr coloured by chain, front view.
PDB entry 6ulr coloured by chain, side view.
PDB entry 6ulr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-affinity oligoclonal TCRs define effective adoptive T cell therapy targeting mutant KRAS-G12D.
Sim MJW, Lu J, Spencer M, Hopkins F, Tran E, Rosenberg SA, Long EO, Sun PD
Proc Natl Acad Sci U S A 117 12826-12835 (2020)
PMID: 32461371

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-231605 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: C1K0Y1 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-C, HLA-Cw
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
GTPase NRas Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 762 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01111 (Residues: 10-18; Coverage: 5%)
Gene names: HRAS1, NRAS
TCR-V-alpha 4*01 Chain: D
Molecule details ›
Chain: D
Length: 206 amino acids
Theoretical weight: 22.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
TCR-V-beta 5-6*01 Chain: E
Molecule details ›
Chain: E
Length: 244 amino acids
Theoretical weight: 27.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 73.031Å b: 73.998Å c: 107.899Å
α: 90° β: 101.458° γ: 90°
R-values:
R R work R free
0.224 0.222 0.262
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

23 review citations

Clinical implications of T cell exhaustion for cancer immunotherapy.
Chow et al. (2022)
22 more

4 mentions without citation

T Cell Recognition of Tumor Neoantigens and Insights Into T Cell Immunotherapy.
Sim et al. (2022)
3 more