› 6ulx

X-ray diffraction
2.31Å resolution

Adenylation domain of a keto acid-selecting NRPS module bound to keto acyl adenylate space group P43212

Released:
DOI: 10.2210/pdb6ulx/pdb
PDB entry 6ulx coloured by chain, front view.
PDB entry 6ulx coloured by chain, side view.
PDB entry 6ulx coloured by chain, top view.
Source organism: Bacillus stratosphericus LAMA 585
Primary publication:
Structural basis of keto acid utilization in nonribosomal depsipeptide synthesis.
Alonzo DA, Chiche-Lapierre C, Tarry MJ, Wang J, Schmeing TM
Nat Chem Biol 16 493-496 (2020)
PMID: 32066969

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241518 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amino acid adenylation domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 576 amino acids
Theoretical weight: 63.94 KDa
Source organism: Bacillus stratosphericus LAMA 585
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand QA7 1 x QA7
Ligand SO4 17 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P43212
Unit cell:
a: 103.274Å b: 103.274Å c: 172.747Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.249
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Nonribosomal peptide synthetases and their biotechnological potential in Penicillium rubens.
Iacovelli et al. (2021)
2 more

2 mentions without citation

Biosynthesis of depsipeptides, or Depsi: The peptides with varied generations.
Alonzo et al. (2020)
1 more