› 6umx

X-ray diffraction
2.79Å resolution

Structural basis for specific inhibition of extracellular activation of pro/latent myostatin by SRK-015

Released:
DOI: 10.2210/pdb6umx/pdb
PDB entry 6umx coloured by chain, front view.
PDB entry 6umx coloured by chain, side view.
PDB entry 6umx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of specific inhibition of extracellular activation of pro- or latent myostatin by the monoclonal antibody SRK-015.
Dagbay KB, Treece E, Streich FC, Jackson JW, Faucette RR, Nikiforov A, Lin SC, Boston CJ, Nicholls SB, Capili AD, Carven GJ
J Biol Chem 295 5404-5418 (2020)
PMID: 32075906

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-127149 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Growth/differentiation factor 8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 41.64 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O14793 (Residues: 24-375; Coverage: 100%)
Gene names: GDF8, MSTN
Sequence domains:
Immunoglobulin lambda constant 2 Chains: L, l
Molecule details ›
Chains: L, l
Length: 215 amino acids
Theoretical weight: 22.66 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DOY2 (Residues: 1-106; Coverage: 100%)
Gene name: IGLC2
Sequence domains: Immunoglobulin C1-set domain
Ig-like domain-containing protein Chains: H, h
Molecule details ›
Chains: H, h
Length: 229 amino acids
Theoretical weight: 24.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q5EFE5 (Residues: 20-248; Coverage: 50%)
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 59.62Å b: 110.01Å c: 293.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.264
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Advances in Hydrogen/Deuterium Exchange Mass Spectrometry and the Pursuit of Challenging Biological Systems.
James et al. (2022)
6 more

4 mentions without citation

Recent advances of IDH1 mutant inhibitor in cancer therapy.
Tian et al. (2022)
3 more