› 6un1

X-ray diffraction
2.26Å resolution

Crystal structure of Pseudomonas aeruginosa PBP3 in complex with temocillin

Released:
DOI: 10.2210/pdb6un1/pdb
PDB entry 6un1 coloured by chain, front view.
PDB entry 6un1 coloured by chain, side view.
PDB entry 6un1 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Influence of the α-Methoxy Group on the Reaction of Temocillin with Pseudomonas aeruginosa PBP3 and CTX-M-14 β-Lactamase.
Sacco MD, Kroeck KG, Kemp MT, Zhang X, Andrews LD, Chen Y
Antimicrob Agents Chemother 64 (2019)
PMID: 31685462

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidoglycan D,D-transpeptidase FtsI Chain: A
Molecule details ›
Chain: A
Length: 533 amino acids
Theoretical weight: 57.64 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: G3XD46 (Residues: 50-579; Coverage: 92%)
Gene names: PA4418, ftsI, pbpB
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand TJ7 1 x TJ7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 67.126Å b: 81.712Å c: 88.082Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.234
Expression system: Escherichia coli

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Citations

3 citation in other articles

Reduced In Vitro Susceptibility of Streptococcus pyogenes to β-Lactam Antibiotics Associated with Mutations in the pbp2x Gene Is Geographically Widespread.
Musser et al. (2020)
2 more

1 mention without citation

Influence of the α-Methoxy Group on the Reaction of Temocillin with Pseudomonas aeruginosa PBP3 and CTX-M-14 β-Lactamase.
Sacco et al. (2019)