› 6unv

X-ray diffraction
3Å resolution

Crystal structure of a methanol tolerant lipase/esterase from the fungus Rasamsonia emersonii

Released:
DOI: 10.2210/pdb6unv/pdb
PDB entry 6unv coloured by chain, front view.
PDB entry 6unv coloured by chain, side view.
PDB entry 6unv coloured by chain, top view.
Source organism: Rasamsonia emersonii
Primary publication:
A Novel Fungal Lipase With Methanol Tolerance and Preference for Macaw Palm Oil.
Rade LL, da Silva MNP, Vieira PS, Milan N, de Souza CM, de Melo RR, Klein BC, Bonomi A, de Castro HF, Murakami MT, Zanphorlin LM
Front Bioeng Biotechnol 8 304 (2020)
PMID: 32435636

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101245 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 31.78 KDa
Source organism: Rasamsonia emersonii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0F4YFS6 (Residues: 22-299; Coverage: 100%)
Gene name: T310_9425
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I4122
Unit cell:
a: 91Å b: 91Å c: 116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.284 0.279 0.329
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Expression of novel acidic lipase from Micrococcus luteus in Pichia pastoris and its application in transesterification.
Adina et al. (2021)
1 more

3 mentions without citation

A Novel Fungal Lipase With Methanol Tolerance and Preference for Macaw Palm Oil.
Rade et al. (2020)
2 more