› 6unw

X-ray diffraction
2.21Å resolution

Epoxide hydrolase from an endophytic Streptomyces

Released:
DOI: 10.2210/pdb6unw/pdb
PDB entry 6unw coloured by chain, front view.
PDB entry 6unw coloured by chain, side view.
PDB entry 6unw coloured by chain, top view.
Source organism: Streptomyces sp. CBMAI 2042
Primary publication:
An epoxide hydrolase from endophytic Streptomyces shows unique structural features and wide biocatalytic activity.
Tormet-González GD, Wilson C, de Oliveira GS, Dos Santos JC, de Oliveira LG, Dias MVB
Acta Crystallogr D Struct Biol 76 868-875 (2020)
PMID: 32876062

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167582 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble epoxide hydrolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 353 amino acids
Theoretical weight: 38.25 KDa
Source organism: Streptomyces sp. CBMAI 2042
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

2 bound ligands:
Ligand CAC 3 x CAC
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P4122
Unit cell:
a: 106.733Å b: 106.733Å c: 233.039Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.227
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

1 mention without citation

An epoxide hydrolase from endophytic Streptomyces shows unique structural features and wide biocatalytic activity.
Tormet-González et al. (2020)