› 6uoj

X-ray diffraction
2.35Å resolution

Crystal structure of cytosolic fumarate hydratase from Leishmania major in a complex with succinate

Released:
DOI: 10.2210/pdb6uoj/pdb
PDB entry 6uoj coloured by chain, front view.
PDB entry 6uoj coloured by chain, side view.
PDB entry 6uoj coloured by chain, top view.
Source organism: Leishmania major strain Friedlin
Primary publication:
Structural and Biochemical Investigations of the [4Fe-4S] Cluster-Containing Fumarate Hydratase from Leishmania major.
Feliciano PR, Drennan CL
Biochemistry 58 5011-5021 (2019)
PMID: 31743022

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123143 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 604 amino acids
Theoretical weight: 66.51 KDa
Source organism: Leishmania major strain Friedlin
Expression system: Escherichia coli
UniProt:
  • Canonical: E9AE57 (Residues: 1-568; Coverage: 100%)
Gene names: FH2, LMJF_29_1960
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand SF4 2 x SF4
Ligand SIN 2 x SIN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 65.61Å b: 84.838Å c: 240.542Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.148 0.202
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Cyanobacterial Dihydroxyacid Dehydratases Are a Promising Growth Inhibition Target.
Zhang et al. (2020)
1 more

1 mention without citation

Structural and Biochemical Investigations of the [4Fe-4S] Cluster-Containing Fumarate Hydratase from Leishmania major.
Feliciano et al. (2019)