› 6uon

X-ray diffraction
3.5Å resolution

Molecular basis for tumor infiltrating TCR recognition of hotspot KRAS-G12D mutation

Released:
DOI: 10.2210/pdb6uon/pdb
PDB entry 6uon coloured by chain, front view.
PDB entry 6uon coloured by chain, side view.
PDB entry 6uon coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-affinity oligoclonal TCRs define effective adoptive T cell therapy targeting mutant KRAS-G12D.
Sim MJW, Lu J, Spencer M, Hopkins F, Tran E, Rosenberg SA, Long EO, Sun PD
Proc Natl Acad Sci U S A 117 12826-12835 (2020)
PMID: 32461371

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-231606 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
TCR-V-alpha-12-02*01 Chains: G, I
Molecule details ›
Chains: G, I
Length: 202 amino acids
Theoretical weight: 22.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
TCR-V-beta-10-2*01 Chains: H, J
Molecule details ›
Chains: H, J
Length: 241 amino acids
Theoretical weight: 27.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Ig-like domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 274 amino acids
Theoretical weight: 31.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: C1K0Y1 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-C, HLA-Cw
Sequence domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
GTPase NRas Chains: C, F
Molecule details ›
Chains: C, F
Length: 10 amino acids
Theoretical weight: 875 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01111 (Residues: 10-19; Coverage: 5%)
Gene names: HRAS1, NRAS

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 65.399Å b: 79.286Å c: 197.71Å
α: 90° β: 91.109° γ: 90°
R-values:
R R work R free
0.253 0.252 0.294
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

22 review citations

Clinical implications of T cell exhaustion for cancer immunotherapy.
Chow et al. (2022)
21 more

6 mentions without citation

TCR Recognition of Peptide-MHC-I: Rule Makers and Breakers.
Szeto et al. (2020)
5 more