› 6upa

X-ray diffraction
2.51Å resolution

Crystal Structure of GTPase Domain of Human Septin 2/Septin 6 Heterocomplex

Released:
DOI: 10.2210/pdb6upa/pdb
PDB entry 6upa coloured by chain, front view.
PDB entry 6upa coloured by chain, side view.
PDB entry 6upa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular Recognition at Septin Interfaces: The Switches Hold the Key.
Rosa HVD, Leonardo DA, Brognara G, Brandão-Neto J, D'Muniz Pereira H, Araújo APU, Garratt RC
J Mol Biol 432 5784-5801 (2020)
PMID: 32910969

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171929 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Septin-6 Chain: B
Molecule details ›
Chain: B
Length: 280 amino acids
Theoretical weight: 32.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14141 (Residues: 40-305; Coverage: 61%)
Gene names: KIAA0128, SEP2, SEPT6, SEPTIN6
Sequence domains: Septin
Septin-2 Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 34.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15019 (Residues: 35-308; Coverage: 76%)
Gene names: DIFF6, KIAA0158, NEDD5, SEPT2, SEPTIN2
Sequence domains: Septin

Ligands and Environments

3 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P41212
Unit cell:
a: 82.73Å b: 82.73Å c: 170.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Cellular functions of actin- and microtubule-associated septins.
Spiliotis et al. (2021)
1 more

1 mention without citation

Characterization of host substrates of SARS-CoV-2 main protease.
Melano et al. (2023)