› 6upm

X-ray diffraction
2.03Å resolution

Crystal structure of T467A variant of cytosolic fumarate hydratase from Leishmania major in a complex with S-malate

Released:
DOI: 10.2210/pdb6upm/pdb
Model geometry
Data not analysed
Fit model/data
Data not analysed
PDB entry 6upm coloured by chain, front view.
PDB entry 6upm coloured by chain, side view.
PDB entry 6upm coloured by chain, top view.
Source organism: Leishmania major strain Friedlin
Primary publication:
Structural and Biochemical Investigations of the [4Fe-4S] Cluster-Containing Fumarate Hydratase from Leishmania major.
Feliciano PR, Drennan CL
OpenAccess logo Biochemistry 58 5011-5021 (2019)
PMID: 31743022

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123143 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 604 amino acids
Theoretical weight: 66.48 KDa
Source organism: Leishmania major strain Friedlin
Expression system: Escherichia coli
UniProt:
  • Canonical: E9AE57 (Residues: 1-568; Coverage: 100%)
Gene names: FH2, LMJF_29_1960
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand SF4 2 x SF4
Ligand LMR 2 x LMR
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 65.616Å b: 84.718Å c: 239.669Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.193
Expression system: Escherichia coli

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Citations

2 citation in other articles

Cyanobacterial Dihydroxyacid Dehydratases Are a Promising Growth Inhibition Target.
Zhang et al. (2020)
1 more

1 mention without citation

Structural and Biochemical Investigations of the [4Fe-4S] Cluster-Containing Fumarate Hydratase from Leishmania major.
Feliciano et al. (2019)