› 6upq

X-ray diffraction
1.86Å resolution

Crystal Structure of GTPase Domain of Human Septin 2 / Septin 11 Heterocomplex

Released:
DOI: 10.2210/pdb6upq/pdb
PDB entry 6upq coloured by chain, front view.
PDB entry 6upq coloured by chain, side view.
PDB entry 6upq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular Recognition at Septin Interfaces: The Switches Hold the Key.
Rosa HVD, Leonardo DA, Brognara G, Brandão-Neto J, D'Muniz Pereira H, Araújo APU, Garratt RC
J Mol Biol 432 5784-5801 (2020)
PMID: 32910969

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172158 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Septin-2 Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15019 (Residues: 36-308; Coverage: 76%)
Gene names: DIFF6, KIAA0158, NEDD5, SEPT2, SEPTIN2
Sequence domains: Septin
Septin-11 Chain: B
Molecule details ›
Chain: B
Length: 293 amino acids
Theoretical weight: 33.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NVA2 (Residues: 40-306; Coverage: 62%)
Gene names: SEPT11, SEPTIN11
Sequence domains: Septin

Ligands and Environments

3 bound ligands:
Ligand GDP 1 x GDP
Ligand GTP 1 x GTP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 56.654Å b: 81.859Å c: 68.221Å
α: 90° β: 97.13° γ: 90°
R-values:
R R work R free
0.188 0.187 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Cellular functions of actin- and microtubule-associated septins.
Spiliotis et al. (2021)
6 other articles

1 mention without citation

The Structural Biology of Septins and Their Filaments: An Update.
Cavini et al. (2021)