› 6upr

X-ray diffraction
2.3Å resolution

Crystal Structure of GTPase Domain of Human Septin 2 / Septin 8 Heterocomplex

Released:
DOI: 10.2210/pdb6upr/pdb
PDB entry 6upr coloured by chain, front view.
PDB entry 6upr coloured by chain, side view.
PDB entry 6upr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular Recognition at Septin Interfaces: The Switches Hold the Key.
Rosa HVD, Leonardo DA, Brognara G, Brandão-Neto J, D'Muniz Pereira H, Araújo APU, Garratt RC
J Mol Biol 432 5784-5801 (2020)
PMID: 32910969

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172157 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Septin-2 Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15019 (Residues: 36-308; Coverage: 76%)
Gene names: DIFF6, KIAA0158, NEDD5, SEPT2, SEPTIN2
Sequence domains: Septin
Septin-8 Chain: B
Molecule details ›
Chain: B
Length: 292 amino acids
Theoretical weight: 33.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92599 (Residues: 36-311; Coverage: 57%)
Gene names: KIAA0202, SEPT8, SEPTIN8
Sequence domains: Septin

Ligands and Environments

3 bound ligands:
Ligand GDP 1 x GDP
Ligand GTP 1 x GTP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 52.754Å b: 82.773Å c: 78.144Å
α: 90° β: 103.63° γ: 90°
R-values:
R R work R free
0.21 0.208 0.255
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

2 review citations

Cellular functions of actin- and microtubule-associated septins.
Spiliotis et al. (2021)
1 more

1 mention without citation

Seeded assembly in vitro does not replicate the structures of α-synuclein filaments from multiple system atrophy.
Lövestam et al. (2021)