› 6uro

Electron Microscopy
3.6Å resolution

Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex

Released:
DOI: 10.2210/pdb6uro/pdb
PDB entry 6uro coloured by chain, front view.
PDB entry 6uro coloured by chain, side view.
PDB entry 6uro coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery.
Zhang Y, Sun Y, Shi Y, Walz T, Tong L
Mol Cell 77 800-809.e6 (2020)
PMID: 31810758
Related structures: EMD-20861

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-131969 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Cleavage and polyadenylation specificity factor subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 1443 amino acids
Theoretical weight: 161.07 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q10570 (Residues: 1-1443; Coverage: 100%)
Gene names: CPSF1, CPSF160
Sequence domains:
pre-mRNA 3' end processing protein WDR33 Chain: B
Molecule details ›
Chain: B
Length: 587 amino acids
Theoretical weight: 67.55 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9C0J8 (Residues: 1-572; Coverage: 43%)
Gene names: WDC146, WDR33
Sequence domains: WD domain, G-beta repeat
Cleavage and polyadenylation specificity factor subunit 4 Chain: C
Molecule details ›
Chain: C
Length: 250 amino acids
Theoretical weight: 28.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O95639 (Residues: 1-269; Coverage: 91%)
Gene names: CPSF30, CPSF4, NAR, NEB1
Sequence domains:
Cleavage stimulation factor subunit 3 Chains: E, F
Molecule details ›
Chains: E, F
Length: 717 amino acids
Theoretical weight: 83.04 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q12996 (Residues: 1-717; Coverage: 100%)
Gene name: CSTF3
Sequence domains: Suppressor of forked protein (Suf)
PAS RNA Chain: D
Molecule details ›
Chain: D
Length: 47 nucleotides
Theoretical weight: 14.82 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-20861
Expression system: Trichoplusia ni

Quick links

Citations

8 review citations

Recent molecular insights into canonical pre-mRNA 3'-end processing.
Sun et al. (2020)
7 more

2 mentions without citation

Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery.
Zhang et al. (2020)
1 more