› 6ux6

X-ray diffraction
1.94Å resolution

Cruzain covalently bound by a vinylsulfone compound

Released:
DOI: 10.2210/pdb6ux6/pdb
PDB entry 6ux6 coloured by chain, front view.
PDB entry 6ux6 coloured by chain, side view.
PDB entry 6ux6 coloured by chain, top view.
Source organism: Trypanosoma cruzi
Entry authors: Zhai X, Tang S, Chenna BC, Meek TD, Sacchettini JC

Function and Biology Details

Reaction catalysed: 
Broad endopeptidase specificity similar to that of cathepsin L.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150579 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cruzipain Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 22.72 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: P25779 (Residues: 123-337; Coverage: 48%)
Sequence domains: Papain family cysteine protease

Ligands and Environments

2 bound ligands:
Ligand TM8 1 x TM8
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21212
Unit cell:
a: 40.509Å b: 122.127Å c: 34.585Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.221 0.268
Expression system: Escherichia coli

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