› 6uxk

X-ray diffraction
2.1Å resolution

Structure of serine hydroxymethyltransferase 8 from Glycine max cultivar Forrest complexed with PLP

Released:
DOI: 10.2210/pdb6uxk/pdb
PDB entry 6uxk coloured by chain, front view.
PDB entry 6uxk coloured by chain, side view.
PDB entry 6uxk coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Impaired folate binding of serine hydroxymethyltransferase 8 from soybean underlies resistance to the soybean cyst nematode.
Korasick DA, Kandoth PK, Tanner JJ, Mitchum MG, Beamer LJ
J Biol Chem 295 3708-3718 (2020)
PMID: 32014996

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 52.39 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0R0IK90 (Residues: 71-541; Coverage: 87%)
Gene names: 100305380, GLYMA_08G108900
Sequence domains: Serine hydroxymethyltransferase

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Unit cell:
a: 56.12Å b: 127.97Å c: 128.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.225
Expression system: Escherichia coli

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Citations

2 review citations

A Broad Review of Soybean Research on the Ongoing Race to Overcome Soybean Cyst Nematode.
Nissan et al. (2022)
1 more

1 mention without citation

Impaired folate binding of serine hydroxymethyltransferase 8 from soybean underlies resistance to the soybean cyst nematode.
Korasick et al. (2020)