› 6uxl

X-ray diffraction
2.35Å resolution

Structure of serine hydroxymethyltransferase 8 from Glycine max cultivar Forrest complexed with PLP-Glycine

Released:
DOI: 10.2210/pdb6uxl/pdb
PDB entry 6uxl coloured by chain, front view.
PDB entry 6uxl coloured by chain, side view.
PDB entry 6uxl coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Impaired folate binding of serine hydroxymethyltransferase 8 from soybean underlies resistance to the soybean cyst nematode.
Korasick DA, Kandoth PK, Tanner JJ, Mitchum MG, Beamer LJ
J Biol Chem 295 3708-3718 (2020)
PMID: 32014996

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125526 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 52.17 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: K4FW35 (Residues: 1-471; Coverage: 100%)
Gene name: SHMT
Sequence domains: Serine hydroxymethyltransferase

Ligands and Environments

1 bound ligand:
Ligand PLG 2 x PLG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Unit cell:
a: 55.727Å b: 127.445Å c: 127.733Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.242
Expression system: Escherichia coli

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Citations

2 review citations

A Broad Review of Soybean Research on the Ongoing Race to Overcome Soybean Cyst Nematode.
Nissan et al. (2022)
1 more

1 mention without citation

Impaired folate binding of serine hydroxymethyltransferase 8 from soybean underlies resistance to the soybean cyst nematode.
Korasick et al. (2020)