› 6v0k

X-ray diffraction
2.41Å resolution

Crystal structure of Penicillium verruculosum copalyl diphosphate synthase (PvCPS) alpha prenyltransferase domain

Released:
DOI: 10.2210/pdb6v0k/pdb
Model geometry
Data not analysed
Fit model/data
Data not analysed
PDB entry 6v0k coloured by chain, front view.
PDB entry 6v0k coloured by chain, side view.
PDB entry 6v0k coloured by chain, top view.
Source organism: Talaromyces verruculosus
Primary publication:
Higher-order oligomerization of a chimeric αβγ bifunctional diterpene synthase with prenyltransferase and class II cyclase activities is concentration-dependent.
Ronnebaum TA, Gupta K, Christianson DW
J Struct Biol 210 107463 (2020)
PMID: 31978464

Function and Biology Details

Reactions catalysed: 
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate
Geranylgeranyl diphosphate = (+)-copalyl diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copalyl diphosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 34.79 KDa
Source organism: Talaromyces verruculosus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A348FUE1 (Residues: 660-963; Coverage: 32%)
Gene name: PvCPS
Sequence domains: Polyprenyl synthetase

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P321
Unit cell:
a: 188.117Å b: 188.117Å c: 56.412Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.219
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Structural insight on assembly-line catalysis in terpene biosynthesis.
Faylo et al. (2021)
3 more

3 mentions without citation

Assembly-Line Catalysis in Bifunctional Terpene Synthases.
Faylo et al. (2021)
2 more