› 6v1c

X-ray diffraction
1.55Å resolution

Crystal structure of human trefoil factor 3 in complex with its cognate ligand

Released:
DOI: 10.2210/pdb6v1c/pdb
PDB entry 6v1c coloured by chain, front view.
PDB entry 6v1c coloured by chain, side view.
PDB entry 6v1c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Trefoil factors share a lectin activity that defines their role in mucus.
Järvå MA, Lingford JP, John A, Soler NM, Scott NE, Goddard-Borger ED
Nat Commun 11 2265 (2020)
PMID: 32404934

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170488 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Trefoil factor 3 Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07654 (Residues: 22-79; Coverage: 98%)
Gene names: ITF, TFF3, TFI
Sequence domains: Trefoil (P-type) domain

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, NDG
Carbohydrate polymer
No bound ligands
1 modified residue:
Ligand MLY 4 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P41212
Unit cell:
a: 37.588Å b: 37.588Å c: 87.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.174 0.188
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Carbohydrate-active enzymes (CAZymes) in the gut microbiome.
Wardman et al. (2022)
6 more

2 mentions without citation

Trefoil factors share a lectin activity that defines their role in mucus.
Järvå et al. (2020)
1 more