› 6v2o

X-ray diffraction
1.27Å resolution

HLA-B*57:01 presenting the peptide ASLNLPAVSW

Released:
DOI: 10.2210/pdb6v2o/pdb
PDB entry 6v2o coloured by chain, front view.
PDB entry 6v2o coloured by chain, side view.
PDB entry 6v2o coloured by chain, top view.
Source organisms:
Primary publication:
The molecular basis of how buried human leukocyte antigen polymorphism modulates natural killer cell function.
Saunders PM, MacLachlan BJ, Pymm P, Illing PT, Deng Y, Wong SC, Oates CVL, Purcell AW, Rossjohn J, Vivian JP, Brooks AG
Proc Natl Acad Sci U S A 117 11636-11647 (2020)
PMID: 32404419

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-152827 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: U6BR87 (Residues: 25-300; Coverage: 88%)
Gene name: HLA-B
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Catenin alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.06 KDa
Source organism: synthetic construct
Expression system: synthetic construct
UniProt:
  • Canonical: P35221 (Residues: 850-859; Coverage: 1%)
Gene name: CTNNA1

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 50.762Å b: 81.95Å c: 110.487Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.134 0.159
Expression systems:
  • Escherichia coli BL21
  • synthetic construct

Quick links

Citations

11 citation in other articles

Killer Cell Immunoglobulin-like Receptor Variants Are Associated with Protection from Symptoms Associated with More Severe Course in Parkinson Disease.
Anderson et al. (2020)
10 more

2 mentions without citation

Unconventional Peptide Presentation by Classical MHC Class I and Implications for T and NK Cell Activation.
Zajonc DM. (2020)
1 more