› 6v4s

X-ray diffraction
3.55Å resolution

A Closed pore conformation of a Pentameic ligand-gated ion channel with additional N-terminal domain

Released:
DOI: 10.2210/pdb6v4s/pdb
Model geometry
Data not analysed
Fit model/data
Data not analysed
PDB entry 6v4s coloured by chain, front view.
PDB entry 6v4s coloured by chain, side view.
PDB entry 6v4s coloured by chain, top view.
Source organism: Desulfofustis sp. PB-SRB1
Primary publication:
Structural basis for allosteric transitions of a multidomain pentameric ligand-gated ion channel.
Hu H, Howard RJ, Bastolla U, Lindahl E, Delarue M
Proc Natl Acad Sci U S A 117 13437-13446 (2020)
PMID: 32482881

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neur_chan_LBD domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 642 amino acids
Theoretical weight: 71.73 KDa
Source organism: Desulfofustis sp. PB-SRB1
Expression system: Escherichia coli
UniProt:
  • Canonical: V4JF97 (Residues: 1-642; Coverage: 100%)
Gene name: JKP90_15375
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain

Ligands and Environments

4 bound ligands:
Ligand CA 5 x CA
Ligand NA 7 x NA
Ligand CL 5 x CL
Ligand LMT 10 x LMT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 159.406Å b: 337.254Å c: 111.812Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.268
Expression system: Escherichia coli

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Citations

1 review citation

Phenols and GABAA receptors: from structure and molecular mechanisms action to neuropsychiatric sequelae.
Menzikov et al. (2024)
5 other articles

4 mentions without citation

Recent Insight into Lipid Binding and Lipid Modulation of Pentameric Ligand-Gated Ion Channels.
Ananchenko et al. (2022)
3 more