› 6v51

X-ray diffraction
1.5Å resolution

Spin-labeled T4 Lysozyme (9/131FnbY)-(4-Amino-TEMPO)

Released:
DOI: 10.2210/pdb6v51/pdb
PDB entry 6v51 coloured by chain, front view.
PDB entry 6v51 coloured by chain, side view.
PDB entry 6v51 coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Genetically Encoded Quinone Methides Enabling Rapid, Site-Specific, and Photocontrolled Protein Modification with Amine Reagents.
Liu J, Cheng R, Van Eps N, Wang N, Morizumi T, Ou WL, Klauser PC, Rozovsky S, Ernst OP, Wang L
J Am Chem Soc 142 17057-17068 (2020)
PMID: 32915556

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.76 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli str. K-12 substr. DH10B
UniProt:
  • Canonical: D9IEF7 (Residues: 1-164; Coverage: 100%)
Gene names: T4Tp126, e
Sequence domains: Phage lysozyme

Ligands and Environments

1 bound ligand:
Ligand QPM 2 x QPM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P3221
Unit cell:
a: 59.407Å b: 59.407Å c: 95.489Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.168 0.184
Expression system: Escherichia coli str. K-12 substr. DH10B

Quick links

Citations

4 review citations

New covalent bonding ability for proteins.
Cao et al. (2022)
3 more

1 mention without citation

Genetically Encoded Quinone Methides Enabling Rapid, Site-Specific, and Photocontrolled Protein Modification with Amine Reagents.
Liu et al. (2020)