› 6v5b

Electron Microscopy
3.7Å resolution

Human Drosha and DGCR8 in complex with Primary MicroRNA (MP/RNA complex) - Active state

Released:
DOI: 10.2210/pdb6v5b/pdb
PDB entry 6v5b coloured by chain, front view.
PDB entry 6v5b coloured by chain, side view.
PDB entry 6v5b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA.
Partin AC, Zhang K, Jeong BC, Herrell E, Li S, Chiu W, Nam Y
Mol Cell 78 411-422.e4 (2020)
PMID: 32220646
Related structures: EMD-21051

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186806 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Ribonuclease 3 Chain: A
Molecule details ›
Chain: A
Length: 1016 amino acids
Theoretical weight: 118.38 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q9NRR4 (Residues: 353-1365; Coverage: 74%)
Gene names: DROSHA, RN3, RNASE3L, RNASEN
Sequence domains:
Microprocessor complex subunit DGCR8 Chains: B, C
Molecule details ›
Chains: B, C
Length: 532 amino acids
Theoretical weight: 60.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8WYQ5 (Residues: 223-751; Coverage: 68%)
Gene names: C22orf12, DGCR8, DGCRK6, LP4941
Sequence domains: Double-stranded RNA binding motif
Pri-miR-16-2 (78-MER) Chain: D
Molecule details ›
Chain: D
Length: 105 nucleotides
Theoretical weight: 33.65 KDa
Sequence domains: mir-16 microRNA precursor family

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-21051
Expression systems:
  • Baculovirus expression vector pFastBac1-HM
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

11 review citations

microRNAs in action: biogenesis, function and regulation.
Shang et al. (2023)
10 more

8 mentions without citation

A Structural View of miRNA Biogenesis and Function.
Leitão et al. (2022)
7 more