› 6v5k

X-ray diffraction
2.69Å resolution

Crystal structure of human DNA polymerase eta complexed with N7-nitrogen half-mustard guanine (NHMG) and dCTP*

Released:
DOI: 10.2210/pdb6v5k/pdb
PDB entry 6v5k coloured by chain, front view.
PDB entry 6v5k coloured by chain, side view.
PDB entry 6v5k coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Jung H, Lee S

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120744 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase eta Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 48.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y253 (Residues: 1-432; Coverage: 61%)
Gene names: POLH, RAD30, RAD30A, XPV
Sequence domains:
DNA (5'-D(*AP*GP*TP*GP*TP*GP*AP*G)-3') Chain: P
Molecule details ›
Chain: P
Length: 8 nucleotides
Theoretical weight: 2.51 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*AP*T*(QRV)P*CP*TP*CP*AP*CP*AP*CP*T)-3') Chain: T
Molecule details ›
Chain: T
Length: 12 nucleotides
Theoretical weight: 3.76 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand 0KX 1 x 0KX
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P61
Unit cell:
a: 98.911Å b: 98.911Å c: 82.138Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.188 0.242
Expression systems:
  • Escherichia coli
  • Not provided

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