› 6v62

X-ray diffraction
2.36Å resolution

SETD3 double mutant (N255F/W273A) in Complex with an Actin Peptide with His73 Replaced with Lysine

Released:
DOI: 10.2210/pdb6v62/pdb
PDB entry 6v62 coloured by chain, front view.
PDB entry 6v62 coloured by chain, side view.
PDB entry 6v62 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An engineered variant of SETD3 methyltransferase alters target specificity from histidine to lysine methylation.
Dai S, Horton JR, Wilkinson AW, Gozani O, Zhang X, Cheng X
J Biol Chem 295 2582-2589 (2020)
PMID: 31911441

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + protein L-histidine = S-adenosyl-L-homocysteine + protein N(tau)-methyl-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159067 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, cytoplasmic 2, N-terminally processed Chain: Y
Molecule details ›
Chain: Y
Length: 23 amino acids
Theoretical weight: 2.86 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P63261 (Residues: 66-88; Coverage: 6%)
Gene names: ACTG, ACTG1
Actin-histidine N-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 596 amino acids
Theoretical weight: 67.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86TU7 (Residues: 1-594; Coverage: 100%)
Gene names: C14orf154, SETD3
Sequence domains:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
1 bound ligand:
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 60.654Å b: 116.752Å c: 174.377Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.252
Expression systems:
  • Not provided
  • Escherichia coli

Quick links

Citations

2 review citations

Enzymology and significance of protein histidine methylation.
Jakobsson ME. (2021)
1 more

1 mention without citation

An engineered variant of SETD3 methyltransferase alters target specificity from histidine to lysine methylation.
Dai et al. (2020)