6v7o

X-ray diffraction
2.9Å resolution

Structural Elucidation of Peptide Binding to KLHL-12, a Substrate Specific Adapter Protein in a Cul3-Ring E3 Ligase Complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-176097 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kelch-like protein 12 Chains: A, B
Molecule details ›
Chains: A, B
Length: 301 amino acids
Theoretical weight: 32.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53G59 (Residues: 268-567; Coverage: 53%)
Gene names: C3IP1, KLHL12
Sequence domains: Kelch motif
Segment polarity protein dishevelled homolog DVL-3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 amino acids
Theoretical weight: 951 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q92997 (Residues: 679-688; Coverage: 1%)
Gene names: DVL3, KIAA0208

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 42.679Å b: 105.124Å c: 63.148Å
α: 90° β: 90.18° γ: 90°
R-values:
R R work R free
0.212 0.207 0.254
Expression systems:
  • Escherichia coli
  • Not provided