› 6vaa

Electron Microscopy
3.4Å resolution

Structure of the Fanconi Anemia ID complex bound to ICL DNA

Released:
DOI: 10.2210/pdb6vaa/pdb
PDB entry 6vaa coloured by chain, front view.
PDB entry 6vaa coloured by chain, side view.
PDB entry 6vaa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DNA clamp function of the monoubiquitinated Fanconi anaemia ID complex.
Wang R, Wang S, Dhar A, Peralta C, Pavletich NP
Nature 580 278-282 (2020)
PMID: 32269332
Related structures: EMD-21134

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120749 (preferred)
Entry contents:
2 distinct polypeptide molecules
4 distinct DNA molecules
Macromolecules (6 distinct):
Fanconi anemia group I protein Chain: A
Molecule details ›
Chain: A
Length: 1328 amino acids
Theoretical weight: 149.57 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NVI1 (Residues: 1-1328; Coverage: 100%)
Gene names: FANCI, KIAA1794
Sequence domains:
Fanconi anemia group D2 protein Chain: B
Molecule details ›
Chain: B
Length: 1451 amino acids
Theoretical weight: 164.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BXW9 (Residues: 1-1451; Coverage: 100%)
Gene names: FACD, FANCD2
Sequence domains: Fanconi anaemia protein FancD2 nuclease
DNA (26-MER) Chain: W
Molecule details ›
Chain: W
Length: 27 nucleotides
Theoretical weight: 8.41 KDa
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: X
Molecule details ›
Chain: X
Length: 18 nucleotides
Theoretical weight: 5.43 KDa
DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chain: Y
Molecule details ›
Chain: Y
Length: 15 nucleotides
Theoretical weight: 4.65 KDa
DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: Z
Molecule details ›
Chain: Z
Length: 16 nucleotides
Theoretical weight: 4.82 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-21134
Expression system: Spodoptera frugiperda

Quick links

Citations

12 review citations

Fanconi anemia: current insights regarding epidemiology, cancer, and DNA repair.
Peake et al. (2022)
11 more

4 mentions without citation

Structural insight into FANCI-FANCD2 monoubiquitination.
Li et al. (2020)
3 more