› 6vap

X-ray diffraction
1.93Å resolution

Structure of the type II thioesterase BorB from the borrelidin biosynthetic cluster

Released:
DOI: 10.2210/pdb6vap/pdb
PDB entry 6vap coloured by chain, front view.
PDB entry 6vap coloured by chain, side view.
PDB entry 6vap coloured by chain, top view.
Source organism: Streptomyces sp. WAC02707
Primary publication:
Structure and Function of BorB, the Type II Thioesterase from the Borrelidin Biosynthetic Gene Cluster.
Curran SC, Pereira JH, Baluyot MJ, Lake J, Puetz H, Rosenburg DJ, Adams P, Keasling JD
Biochemistry 59 1630-1639 (2020)
PMID: 32250597

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105079 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase thioesterase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 266 amino acids
Theoretical weight: 29.39 KDa
Source organism: Streptomyces sp. WAC02707
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A454WD44 (Residues: 1-264; Coverage: 100%)
Gene name: EF919_13485
Sequence domains: Thioesterase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2221
Unit cell:
a: 72.45Å b: 92.36Å c: 150.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.25
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Module-Based Polyketide Synthase Engineering for de Novo Polyketide Biosynthesis.
Nava et al. (2023)
2 other articles

1 mention without citation

Thioesterase enzyme families: Functions, structures, and mechanisms.
Caswell et al. (2022)